Fig. 1
CaSO4 promotes MSC migration in a concentration-dependent manner. a, b Wound healing or scratch assay used to measure MSC migration response to different CaSO4 concentrations (3–15 mM) after 24 hours. Results shown as average of four different experiments with six replicates for each condition. A representative image displayed for each condition. c, d Agarose spot assay used to confirm the effect of CaSO4 (3 mM) on MSC migration compared to control (PBS). Results presented as percentage of invaded area and number of migrated cells. Six replicates were performed. e, f CaSO4 (3–5 mM) migration effect was completely abolished when calcium was chelated by the addition of equal EDTA concentrations. Data presented as mean ± SEM. Differences considered significant at *p < 0.05, **p < 0.01, and ***p < 0.001. EDTA ethylenediaminetetraacetic acid