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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Tissue source determines the differentiation potentials of mesenchymal stem cells: a comparative study of human mesenchymal stem cells from bone marrow and adipose tissue

Fig. 2

Difference in osteogenesis ability between ATSCs and BMSCs in vitro. ad Total RNA extracted from ATSCs and BMSCs or cells subjected to OIM for 7 days. Relative expression levels of ALP, Runx2, OPN, and OCN checked by qRT-PCR. β-actin as an internal control. Data expressed as mean ± SD (n = 3). *p < 0.05, compared with MSCs in OIM; #p < 0.05, compared with MSCs in α-MEM. e, f Alizarin Red S staining of calcium deposits formed by ATSCs and BMSCs. ATSCs and BMSCs cultured in OIM for 14 days, then cells fixed and stained with Alizarin Red S. ALP: alkaline phosphatase, ATSC: adipose tissue-derived MSC, BMSC: bone marrow-derived MSC, MEM: minimum essential medium, MSC: mesenchymal stem cell, OCN: Osteocalcin, OIM: osteogenic induction medium, OPN: Osteopontin, Runx2: runt-related transcription factor 2

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