Fig. 2

miR-133 enhanced the survival of MSCs under hypoxic conditions. Apoptosis analyzed by Annexin V/PI staining and flow cytometry. a Scatter diagram of apoptosis in MSCs transfected with miR-133 agomir, antagomir, or their corresponding negative control (NC). b Histogram of apoptosis in different groups. c Western blot analysis of PARP protein in MSCs transfected with different miRNAs. d Cell cycle analyzed by PI staining and flow cytometry. e Histogram of western blot data from (c). f Cell viability detected by CCK8 assay (n = 5). g Cell proliferation detected using CFSE at 24 h after transfection with miRNAs. *p < 0.05, **p < 0.01, ***p < 0.001. CFSE carboxyfluorescein diacetate succinimide ester, CON control, NS not significant, PARP poly ADP-ribose polymerase, PI propidium iodide