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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Pentosan polysulfate binds to STRO-1+ mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

Fig. 3

Fluorescence microscopy images of MPCs (6000/well) cultured with 2.5 μg/ml PPS-FITC for 24 h, fixed in Histochoice MB/ethanol and stained with propidium iodide. a A 2-s exposure image of the MPCs outlined by the low-level background autofluorescence and the higher emission arising from the PPS-FITC located within the cells (arrows). b The same field as a, but with 60-s exposure with excitation at 535 nm confirming the presence of PPS-FITC within the nucleus of the MPCs (arrows). FITC fluorescein isothiocyanate, PPS pentosan polysulfate

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Stem Cell Research & Therapy

ISSN: 1757-6512