Pentosan polysulfate binds to STRO-1+ mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

Wu, Jiehua; Shimmon, Susan; Paton, Sharon; Daly, Christopher; Goldschlager, Tony; Gronthos, Stan; Zannettino, Andrew C. W.; Ghosh, Peter

doi:10.1186/s13287-017-0723-y

Stem Cell Research & Therapy

Table 2 Gene expression changes induced by 24-h cultures of MPCs with 5.0 μg/ml PPS relative to identical cultures of non-primed MPCs

From: Pentosan polysulfate binds to STRO-1⁺ mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

Gene	Fold change	Regulation	Primary functions^a
ACTA2*	1.21	Down	Encoding actin-2 protein a member of the actin family which collectively are responsible for cell motility, structure and integrity.
ADAMTSL4	0.77	Down	Encodes the protein ADAMTS4 which lacks a C-terminal TS motif but when proteolytically processed generates the mature proteinase that degrades aggrecan, a major component of hyaline cartilage.
ANK1*	2.79	Up	Encoding the protein Ankyrin-1 a member of the Ankyrin family that play key roles in cell motility, activation, and proliferation.
COL11A1*	1.21	Down	Encoding one of the alpha chains of type XI collagen.
COL5A3*	2.22	Up	Encodes one of the alpha chains of type V collagen
COMP*	1.08	Down	COMP gene provides the instructions for making the COMP protein, an important regulatory component of the extracellular matrix.
DACT1	1.28	Down	Encodes a protein member of the Dapper family. It interacts with and positively regulates dishevelled-mediated signalling pathways during development and is an antagonist of beta-caterin.
ENPP1	0.58	Down	Encoded protein is type II transmembrane glycoprotein that cleaves a variety of substrates including phosphodiester bonds of nucleotides.
FLG*	1.82	Down	The FLG gene provides instructions for making the large protein profilaggrin.
GREM2	0.64	Down	Encodes a member of the BMP antagonist family likely by binding to BMPs
HSPB7*	1.56	Down	Encodes a member of the heat shock beta-7 protein family
LARGE	0.94	Down	Encodes members of the N-acetylglucosamine-l-transferase protein family responsible for glycosylation of glycoproteins and glycosphingolipids.
LMOD1	0.52	Down	Encodes the leicmodin 1 protein that has a putative membrane-spanning region and two types of tandemly repeat blocks.
LOXL4	0.64	Down	Encodes a member of the lysyl oxidase family essential for the biogenesis of crosslinks of matrix collagens and elastins.
LRRC15	0.76	Down	Encodes the leucine rich repeat containing 15 protein that constitute regions of the small proteoglycans.
MRVI1	0.83	Down	Encoding protein MRVI1, a substrate of cGMP-dependent kinase-1(PKG1).
SCUBE3	0.86	Down	Encodes Signal peptide-CUB and EGF-like Domain-containing Protein3.
SVIL	0.64	Up	Encodes the protein Supervillin which is tightly associated with actin filaments and plasma membranes.
SYNPO2	1.06	Down	Encodes Synaptodin 2-like protein, GO annotations include actin binding.
TM4SF1	0.72	Up	Encodes a member of the transmembrane 4 superfamily that mediate signal transduction in the regulation of development, activation, and growth.

^a From Gene Cards Human Gene Database Index, Weizmann Institute of Science, 234 Herzi Street, Rehovat 7610001, Israel
* Confirmed via alternative analysis (Star/DESeq)
ADAMTS a disintegrin-like metalloproteinase with thrombospondin motifs, COMP cartilage oligomeric matrix protein, GO gene ontology, MPC mesenchymal progenitor cell, PPS pentosane polysulfate

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ISSN: 1757-6512

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