Fig. 4
Expression of Smad7 is downregulated by hypoxia in hTSCs. a The predicted hypoxia response elements (HRE) were identified in the promoter region of Smad7. Wild type (Wt-HRE-LUC) and mutated (Mut-HRE-LUC) HRE sequences from Smad7 promoter were cloned, respectively, to the 5′-prime of the luciferase reporter (LUC). b hTSCs incubated with media containing 0 (normoxia) or 100 μM (hypoxia) CoCl2, respectively, were analyzed by ChIP assay using IgG control or HIF1α antibody (Ab). c LUC activities of Wt-HRE-LUC or Mut-HRE-LUC constructs described in a were measured in hTSCs under normoxic or hypoxic conditions, respectively. mRNA (d) and protein (e) levels of Smad7 were analyzed in hTSCs under normoxic or hypoxic conditions, respectively. Data are shown as mean ± SD. Student’s t test was utilized for pairwise comparison. *p < 0.05, **p < 0.01, versus normoxia. ns not significant