Table 1 Primer sequences for qRT-PCR
Gene | Primer sequences |
|---|---|
GAPDH | Forward:5′-ACCACAGTCCATGCCATCAC-3′ |
Reverse:5′-TCCACCACCCTGTTGCTGTA-3′ | |
COL2 | Forward:5′- CTCAAGTCGCTGAACAACC -3′ |
Reverse:5′- CTATGTCCACACCAAATTCC -3′ | |
Aggrecan | Forward:5′-AGGATGGCTTCCACCAGTGC-3′ |
Reverse:5′-TGCGTAAAAGACCTCACCCTCC-3′ | |
COL1 | Forward:5′- ATGTTCAGCTTTGTGGAC -3′ Reverse:5′- GGATGCCATCTTGTCCAG -3′ |
SOX9 | Forward:5′- GACGTGCAAGCTGGGAAAGT-3′ |
Reverse:5′- CGGCAGGTATTGGTCAAACTC-3′ |