Fig. 3

Mean cell area variability in MSCs. Representative graphs of the size distributions of MSCs according to a cell area and b log cell area, acquired from filtered data. Maximum value in y-axis (probability) for passage p5 and senescent passage pSEN graphs was set lower than in passage p1 and 3 graphs for better visualization of the size distribution. The two-sample Kolmogorov-Smirnov test was used to verify the similarity between the distributions for passage 1 against the passages 3, 5, and SEN. The p values were all well below any sensible rejection limits, starting from 1.4 × 10^–5 for the passage 3 distributions. Thus, we can safely reject the hypothesis that the distributions between the passages would be the same. The non-parametric Kolmogorov-Smirnov test will give the same p values for the original (a) and the log-transformed (b) distributions