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Table 1 Parameters with explanations acquired using automated image analysis

From: Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures

Size

 Length

Length of image-aligned bounding box of cell

 Width

Width of image-aligned bounding box of cell

 Area

Area of the cell

 Perimeter

Perimeter of the cell

Shape

 Perimeter to area ratio

The ratio of the cell perimeter to 4π area of the cell. A measure of the roundness of the cell.

 Length to width ratio

The ratio of the length and width of the bounding box of the cell.

 Boxed frame ratio

The ratio of the cell area to the bounding box area.

 Convex hull area ratio

Ratio of convex hull (“rubber band bounding the cell”) area to the cell area. Convex hull area ratio is greater in cells with long pointy processes compared to cells with no sharply shaped processes.

 Convex hull perimeter ratio

The ratio of convex hull length to the detected cell edge length.

  1. Altogether nine parameters describing cell morphology were measured using the Cell Insight platform