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Table 2 Mean cell areas, corresponding population doubling numbers, and fold-changes in comparison to passage 1

From: Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures

Population doubling (passage)

Mean cell area (ÎĽm2)

Fold-change in cell area in comparison to passage 1

19.3 ± 1.5 (p1, n = 6)

1827 ± 329

 

28.3 ± 2.1 (p3, n = 6)

2352 ± 386

1.3

33.5 ± 4.7 (p5, n = 6)

4198 ± 1628

2.3

36.6 ± 7.1 (pSEN, n = 5)

8744 ± 2494

4.8

  1. Measurements from passages (p)1, 3, and 5 are performed from all six donors. Morphological analysis of passages 6–9 (pSEN) were acquired from n = 5 due to early senescence of donor MSC-2 cells