Fig. 2

The TSC-mTOR pathway regulates the expression of HLA-DR but not HLA-ABC in IFN-γ-treated MSCs. Flow cytometry analysis of human leukocyte antigen (HLA)-ABC (a) or HLA-DR (b) positive cell proportion (left) and mean fluorescence intensity (MFI; right) at 1, 3, and 5 days after pretreatment with rapamycin (RAPA) for 4 h. Cells without pretreatment with rapamycin were indicated as control. c,d MSCs were pretreated with rapamycin followed by treatment with 10 ng/ml interferon gamma (IFN-γ) for 1, 3, and 5 days. The HLA-ABC (c) or HLA-DR (d) positive cell proportion (left) and MFI (right) were assessed by flow cytometry. Cells without treatment with rapamycin and IFN-γ were indicated as control. e MSCs without or with tuberous sclerosis complex (TSC)2 knockdown were treated with 10 ng/ml IFN-γ for 0, 3, and 5 days. HLA-DR positive cell proportion (left) and MFI (right) were assessed by flow cytometry. Data represent mean ± SD of at least four independent experiments. *p < 0.05. shNC lentivirus carrying scrambled shRNA