Fig. 2

Characterization of MSC fractionated secretome. SDS-PAGE gel for MSC cell lysate (LYS) and exosome-rich fractionated MSC secretome (EXO) (a). DLS data showing average z-value representative of average particle size (b). Confocal microscopic image at lower (×20) and higher (×100) magnification (inset) (scale bar: 1 μm) (c): (i) scanning electron microscopic image (scale bar: 1 μm), (ii) transmission electron microscopic image (scale bar: 1 μm) and (iii) for EFS. Flow cytometric analysis for presence of exosomal marker CD63 in the fractionated secretome (d). Western blot analysis showing presence of exosomal marker CD81 (e). Standard curve for sandwich ELISA performed to confirm presence of exosomal marker CD9 in the fractionated MSC secretome (f). FSC forward scatter