Fig. 3

Treatment of BV2 using conditioned media (CM) from spheroids. a Mesenchymal stem cells (MSCs) were expanded and cultured in 3D to form spheroids. After interleukin (IL)-1 priming, conditioned media from spheroids were collected and added to BV2 cells previously treated with lipopolysaccharide (LPS). Human (h) and murine (m) cytokines were analysed from supernatants 24 h later. Measurements of human cytokines in the conditioned media (b–d) and in the supernatant from treated BV2s (e–g). Murine cytokines were also analysed from the supernatants (h–j). Results indicate that priming spheroids increased the secretion of IL-6 and granulocyte-colony stimulating factor (G-CSF). Analysis of the supernatant of the co-culture revealed a significant decrease in murine tumour necrosis factor (mTNF)-α after treatment with CM from primed spheroids. Dashed lines in c and f indicate the quantification limit for TNF-α ELISA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. nd not detectable, P passage, PBS phosphate-buffered saline, unt untreated