Fig. 2

The inhibitory effect of autophagy on huMSC function in vitro. a Representative cropped Western blots of Beclin 1, ATG7, LC3, P62, Sox2, stem cell-derived factor-1 (SDF-1), cleaved caspase-3 (CCapase-3), and cleaved poly-ADP-ribose polymerase (CPARP). b Statistical analysis of autophagy-related proteins Beclin 1, ATG7, LC3, and P62 (n = 3). c Statistical analysis of migration- and differentiation-related proteins Sox2 and SDF-1 (n = 3). d Statistical analysis of apoptosis-related proteins Capase-3 and PARP (n = 3). e,f LC3 and CCaspase-3, P62, and Sox2 were co-localized by double immunofluorescence staining, respectively. All data are expressed as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, vs. shNC