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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells

Fig. 2

dCas9-E expression in BM-MSCs. a Bone marrow-derived mesenchymal stem cells (BM-MSCs) were transfected with pLKO.1-puro-U6 and dCas9-E, and stained with PE-labeled anti-HA and DAPI 72 h post-transfection. b The expression of designed dCas9-E nucleases. Scale bar = 50 μm. DAPI 4’6-diamidino-2-phenylindole, GAPDH glyceraldehyde-3-phosphate dehydrogenase, HA hemagglutinin, sgRNA single-guide RNA

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Stem Cell Research & Therapy

ISSN: 1757-6512