Table 1 Primary antibodies used for immunostaining and FACS
Antibody | Company | Catalog no. | Dilution |
|---|---|---|---|
Anti-AFP | Sigma | A-8452 | 1:2000 |
Anti-Chromogranin A | Sigma | MA5-14536 | 1:4000 |
Anti-FOXA2 | Cell Signaling | 3143 | 1:200 |
Anti-Ki67 | BD Pharmingen | 561126 | 1:50 |
Anti-NGN3 | R&D Systems | AF3444 | 1:1000 |
Anti-NKX6.1 | DSHB | F55A12 | 1:2000 |
Anti-NKX6.1 | R&D Systems | LS-C124275 | 1:100 |
Anti-NKX2.2 | DSHB | 74.5A5 | 1:2000 |
Anti-OCT4 | Cell Signaling | C30A3 | 1:500 |
Anti-PDX1 | Abcam | ab47308 | 1:1000 |
Anti-PDX1 | Abcam | ab7383 | 1:100 |
Anti-SOX9 | R&D Systems | AF3075 | 1:500 |
Anti-SOX17 | OriGene | CF500096 | 1:2000 |