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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro

Fig. 5

Effects of hucMSC-CM on expression of CD3, CD68, MCP-1, and TNF-α in each group 14 days postoperatively. A Immunohistochemical staining of kidney tissue sections using CD3 (a–d), CD68 (e–h), MCP-1 (i–l), and TNF-α (m–p). B–E CD3-positive and CD68-positive cells under high-power field (HPF) and total IOD for MCP-1 and TNF-α in each group. F Western blot analysis of CD68, MCP-1, and TNF-α protein in each group. G–I Relative protein expression levels in kidney tissues. β-actin used as inner reference and quantified using densitometric analysis. Values presented as mean ± SD. UUO group compared with Sham, Control, and hucMSC-CM groups. Values statistically significant at: *P < 0.05, Sham and Control groups compared with UUO and hucMSC-CM groups; #P < 0.05. CM conditioned medium, hucMSC human umbilical cord-derived mesenchymal stem cell, IOD integrated optical density, MCP-1 monocyte chemotactic protein 1, TNF tumor necorsis factor, UUO unilateral ureteral obstruction

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Stem Cell Research & Therapy

ISSN: 1757-6512