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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro

Fig. 6

Effects of hucMSC-CM on expression of TLR4, NF-κB, and IκBα in each group 14 days postoperatively. A Immunohistochemical staining of kidney tissue sections using TLR4 (a–d) and p-NF-κB (e–h). B, C Total IOD for TLR4 and p-NF-κB in each group. D Western blot analysis of TLR4, NF-κB, p-NF-κB, IκBα, and p-IκBα protein in each group. E–G Relative protein expression levels in kidney tissues. β-actin used as inner reference and quantified using densitometric analysis. Values presented as mean ± SD. UUO group compared with Sham, Control, and hucMSC-CM groups. Values statistically significant at: *P < 0.05, Sham and Control groups compared with UUO and hucMSC-CM groups; #P < 0.05. CM conditioned medium, hucMSC human umbilical cord-derived mesenchymal stem cell, IOD integrated optical density, p-NF-κB phosphorylated nuclear factor-κB, TLR4 Toll-like receptor 4, UUO unilateral ureteral obstruction

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Stem Cell Research & Therapy

ISSN: 1757-6512