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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro

Fig. 8

a, b Effects of hucMSC-CM on inflammatory cytokines secreted by NRK-52E in each group at 48 hours. hucMSC-CM inhibited the increase of TNF-α and MCP-1 secreted by TGF-β1-induced NRK-52E cells. c, d Immunofluorescent staining for TLR4 and p-NF-κB in each group. e Western blot analysis of TLR4, NF-κB, p-NF-κB, IκBα, and p-IκBα protein in each group. f–h Relative protein expression levels in NRK-52E cells. β-actin used as an inner reference and quantified using densitometric analysis. Values presented as mean ± SD. TGF-β1 group compared with Control and hucMSC-CM groups. Values statistically significant at: *P < 0.05, Control group compared with TGF-β1 and hucMSC-CM groups; #P < 0.05. CM conditioned medium, DAPI 4′,6-diamidino-2-phenylindole hucMSC human umbilical cord-derived mesenchymal stem cell, IOD integrated optical density, MCP-1 monocyte chemotactic protein 1, p-NF-κB phosphorylated nuclear factor-κB, TGF-β1 transforming growth factor β1, TLR4 Toll-like receptor 4, TNF tumor necrosis factor, UUO unilateral ureteral obstruction

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Stem Cell Research & Therapy

ISSN: 1757-6512