Fig. 4

Tet2 knockout hematopoietic precursors show distinct division pattern. a The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP⁺ precursors from wild-type (WT) mouse is shown. The data are a summary from three independent experiments. Error bars represent standard error of the mean (SEM). b The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of wild-type GFP⁺ co-cultured with OP9 stromal cells is shown. OP9 stromal cells were plated in the culture dish 24 h before the time-lapse experiment. Then GFP⁺ hematopoietic precursors were sorted and plated on OP9 stromal cells for tracking of the division of HSCs. The data are a summary from three independent experiments. Error bars represent SEM. c The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of Tet2 knockout (KO) GFP⁺ cells is shown. The data are a summary from three independent experiments. Error bars represent SEM. d The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of Tet2 knockout GFP⁺ cells co-cultured with OP9 stromal cells is shown. OP9 stromal cells were plated in the culture dish 24 h before the time-lapse experiment. Then GFP⁺ hematopoietic precursors were sorted and plated on OP9 stromal cells for tracking of the division of HSCs. The data are a summary from three independent experiments. Error bars represent SEM. e The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of Tet2 knockout GFP⁺ cells treated with 5-aza-2′-deoxycytidine (DAC) 500 nM is shown. The data are a summary from three independent experiments. Error bars represent SEM