Fig. 3

Engraftment of GFP⁺ BMSCs during wound healing. a Second harmonic imaging (SHG) of control (left), BMSC sheets induced without curcumin (CS-N; middle), and BMSC sheets induced by curcumin (CS-C; right) transplanted into the wound site at 28 days and 35 days. Green and red indicate GFP⁺ mesenchymal stem cells (MSCs) and collagen, respectively. b The contents of GFP⁺ BMSCs represented by DNA agarose gel electrophoresis; each lane shows control, CS-N, and CS-C, respectively, at 0 days (lanes 1, 2, 3), 28 days (lanes 4, 5, 6), and 35 days (lanes 7, 8, 9). c Relative quantitative analysis of the content of GFP DNA (n = 4 mice/group). d Ratio of green fluorescent protein (GFP) DNA level at 28 days and 35 days to 0 days. e Sequence alignment results for the amplified products with GFP sequences. Student’s t test (d) and ANOVA (c): *P < 0.05, **P < 0.01, ***P < 0.001