Curcumin-mediated bone marrow mesenchymal stem cell sheets create a favorable immune microenvironment for adult full-thickness cutaneous wound healing

Table 1 Primers used for real-time polymerase chain reaction

Gene	Primer sequence
Col-1	Forward 5’- AAGAAGACATCCCTGAAGTCA -3’
Col-1	Reverse 5’- GCAGATACAGATCAAGCATACC -3’
Col-3	Forward 5’- GCAAGGCAATGAGACTACC -3’
Col-3	Reverse 5’- CCAATGTCCACACCAAATTC -3’
Il-4	Forward 5’- CTAGTTGTCATCCTGCTCTTCT -3’
Il-4	Reverse 5’- CTTCTCCTGTGACCTCGTTC -3’
Tnfα	Forward 5’- AGGTTCTCTTCAAGGGACAA -3’
Tnfα	Reverse 5’- CCTGGTATGAGATAGCAAATCG -3’
Ifnγ	Forward 5’- ATGAACGCTACACACTGC -3’
Ifnγ	Reverse 5’- CCACATCTATGCCACTTGAG -3’
Relmα	Forward 5’- TACTGGGTGTGCTTGTGGCTTTGC -3’
Relmα	Reverse 5’- GGCAGTGGTCCAGTCAACGAGTAAG -3’
Actinβ	Forward 5’- CGTTGACATCCGTAAAGACC -3’
Actinβ	Reverse 5’- TAGGAGCCAGAGCAGTAATC -3’
Cxcr4	Forward 5’- CTCCTCCTGACTATACCTGAC-3’
Cxcr4	Reverse 5’- CGAGACCCACCATTATATGC -3’
Tgfβ	Forward 5’- CTGCTGACCCCCACTGATAC -3’
Tgfβ	Reverse 5’- AGCCCTGTATTCCGTCTCCT -3’
IL1RN	Forward 5’- ACCTTCATAGTGTGTTCTTGG -3’
IL1RN	Reverse 5’- CTTCTTCTTTGTTCTTGCTCAG -3’
Arg1	Forward 5’- GAAGAATGGAAGAGTCAGTGTG -3’
Arg1	Reverse 5’- GGAGTGTTGATGTCAGTGTG -3’
Jag2	Forward 5’- GTGTGGTTATCTGCGTATGG -3’
Jag2	Reverse 5’- GTTGCGGATGGGATTGAG -3’
iNOS	Forward 5’- CCTATCTCCATTCTACTACTACCA G -3’
iNOS	Reverse 5’- ACCACTTTCACCAAGACTCTA -3’
Tbx21	Forward 5’- CGCATCTGTTGATACGAGTG -3’
Tbx21	Reverse 5’- TGGTTGGATAGAAGAGGTGAG -3’

ISSN: 1757-6512