Table 3 Primer sequences
From: Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis
Species | Lineage | Primer | Sequence | Accession number |
|---|---|---|---|---|
Equine | Reference | GAPDH | Forward: AAGAAGGTGGTGAAGCAGGReverse: CTCAGTGTAGCCCAGGATG | NM_001163856.1 |
Osteogenesis | ALP | Forward: GGAGTATGAGATGGACGAGReverse: GTAGTGAGAGTGCTTGTGCC | XM_005607380.2 | |
OCN | Forward: TGGCCCTGACTGCATTCTGReverse: CCCTCCTGCTTGGACATGAA | XM_005610022.1 | ||
OPG | Forward: CCCCCCTTGTCCTGACCACTReverse: CGCCCTTCCTCACATTCG | XM_001916099.4 | ||
BSP | Forward: GAAGAATCGGACGCTGAGReverse: ATCGTAGACAGGGTGGTG | XM_001496125.4 | ||
Murine | Reference | GAPDH | Forward: ACCCAGAAGACTGTGGATGGReverse: ACACATTGGGGGTAGGAACA | XM_017321385.1 |
Osteogenesis | ALP | Forward: TGATGTGGAATACGAACTGGReverse: TGGGAATGCTTGTGTCTG | XM_006538500.2 | |
OCN | Forward: CCATCTTTCTGCTCACTCTGReverse: TTATTGCCCTCCTGCTTG | NM_007541.3 | ||
OPG | Forward: CACTCGAACCTCACCACAGAReverse: GCTCGATTTGCAGGTCTTTC | NM_008764.3 | ||
BSP | Forward: CAGCCATGAGTCAAGTCAGCReverse: CTTGTGGCTCTGATGTTCCA | NM_001204203.1 |