Fig. 1

Coculture of marrow fibroblasts with engineered vessels create perfusable marrow microenvironments. a Schematic of microvessel platform shows a perfusable network that is then b perfused with hematopoietic cells. c Perfused hematopoietic cells are identified as migrated (1, 3) or adhered (2) within a portion of the vessel network. d Quantification of percent vessel coverage by human mesenchymal stem cells (hMSCs) and marrow fibroblast types show that hMSCs and HS27a cells coat vessels more than HS5 fibroblasts. ****p < 0.0001, **p < 0.005. e Cocultured hMSCs and marrow fibroblasts interact with endothelial vessel walls. Scale bars = 100 μm. f Three-dimensional reconstruction and orthogonal views of co-cultured microvessels show surface renderings of MSCs, HS5, and HS27a cells interacting with the endothelial wall. Scale bars = 50 μm