Fig. 6

Effect of DOT1L knockdown on pluripotent and cardiac specific transcripts by qRT-PCR analysis in (a) KIND1 and (b) HES3 cells. Relative expression for pluripotency (OCT4) and cardiac specific (MESP1, MEF2C, NKX2.5, ISL1, CTNT) transcripts during differentiation of KIND1 and HES3 cells, normalized to internal control RPLPO and presented as fold change of expression. Nonpatterned bars represent expression levels in cells with nontarget siRNA while patterned bars represent expression in cells after DOT1L siRNA treatment. Error bars represent ±SEM of three independent knockdown experiments. Statistical significance: *p < 0.5, **p < 0.01, ***p < 0.001