Table 2 Inflammatory-related gene primer pairs in RAW cells used in quantitative reverse transcription-polymerase chain reaction

TNF-α

Forward: 5′-CTGAACTTCGGGGTGATCGG-3′

Reverse: 5′-GGCTTGTCACTCGAATTTTGAGA-3′

IL-1β

Forward: 5′-TGGAGAGTGTGGATCCCAAG-3′

Reverse: 5′-GGTGCTGATGTACCAGTTGG-3′

IFN-γ

Forward: 5′-TGAAAATCCTGCAGAGCCAG-3′

Reverse: 5′-TGGACCTGTGGGTTGTTGAC-3′

IL-6

Forward: 5′-ATAGTCCTTCCTACCCCAATTTCC-3′

Reverse: 5′-GATGAATTGGATGGTCTTGGTCC-3′

IL-10

Forward: 5′-GAGAAGCATGGCCCAGAAATC-3′

Reverse: 5′-GAGAAATCGATGACAGCGCC-3′

IL-1ra

Forward :5′-CTCCAGCTGGAGGAAGTTAAC-3′

Reverse: 5′-CTGACTCAAAGCTGGTGGTG-3′

Arg-1

Forward: 5′-CATATCTGCCAAGGACATCG-3′

Reverse: 5′-GGTCTCTTCCATCACTTTGC-3′

BMP-2

Forward: 5′-GCTCCACAAACGAGAAAAGC-3′

Reverse: 5′-AGCAAGGGGAAAAGGACACT-3′

OSM

Forward: 5′-AACTCTTCCTCTCAGCTCCT-3′

Reverse: 5′-TGTGTTCAGGTTTTGGAGGC-3′

VEGF

Forward: 5′-GTCCCATGAAGTGATCAAGTTC-3′

Reverse: 5’-TCTGCATGGTGATGTTGCTCTCTG-3′

TGF-β1

Forward: 5′-CAGTACAGCAAGGTCCTTGC-3′

Reverse: 5′-ACGTAGTAGACGATGGGCAG-3′

TGF-β3

Forward: 5′-CAACACCCTGAACCCAGAG-3′

Reverse: 5′-CTTCACCACCATGTTGGACAG-3′

GAPDH

Forward: 5′-CTCCCACTCTTCCACCTTCG −3′

Reverse: 5′-TTGCTGTAGCCGTATTCATT −3′