Fig. 1

Microtubule-based cytoskeleton remodeling during adipocyte differentiation (AD) and expression of microtubules (MTs) are induced during adipogenesis. a A schematic drawing depicting morphological changes in the MT network during adipocyte differentiation. Lipid droplets (LDs), MTs, and nuclei are indicated by green circles, red lines, and black circles, respectively. b Scheme of LINC complex remodeling during adipocyte differentiation. INM, inner nuclear membrane; ONM, outer nuclear membrane. c hASCs at days 0, 4, 7, and 14 after adipogenic cocktail treatment were fixed, costained with anti-α-tubulin (red) and for DNA (4′,6-diamidino-2-phenylindole (DAPI); blue) and imaged using a confocal microscope for visualizing MTs and nuclei. Scale bars = 10 μm. d α-tubulin mRNA expression in hASCs at different days postinduction of adipogenesis was quantified by RT-qPCR; **P < 0.01, ***P < 0.001, one-way ANOVA. e, f Whole-cell lysates of differentiation-induced hASCs were analyzed by Western blotting and probed with anti-α-tubulin antibody and for the adipogenic markers C/EBPα and PPARγ. GAPDH was used to ensure equal loading. The graph shows average α-tubulin band intensities normalized to GAPDH; ***P < 0.001, one-way ANOVA