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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Cross-talk between microtubules and the linker of nucleoskeleton complex plays a critical role in the adipogenesis of human adipose-derived stem cells

Fig. 7

LINC complex disruption perturbs the perinuclear organization of microtubules (MTs) in hASCs. a Immunofluorescence analysis of sun2-knockdown hASCs and GFP controls. Cells were stained for α-tubulin (red), SUN2 (cherry), and DNA (4′,6-diamidino-2-phenylindole (DAPI); blue). The boxed area shows the disturbed perinuclear MT network organization. Scale bar = 20 μm. b Model of the effect of sun2 loss on cytoskeletal organization in vitro. In siControl cells, MTs appeared to be shorter, sparser, and had a vacuolar structure. In siSUN2 cells, MTs maintained their integrity and rarely showed disruption. c RT-qPCR analysis of PPARγ, C/EBPα, and α-tubulin. Tests were performed to compare small interfering (si)Control and siSUN2-treated samples at the same time points; **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512