Fig. 5From: Inflammatory licensed equine MSCs are chondroprotective and exhibit enhanced immunomodulation in an inflammatory environmentInflammatory primed MSC secretomes do not induce inflammatory changes in untreated chondrocytes. To analyze functional significance of inflammatory primed MSCs on cartilage health, inflammatory primed MSCs were cultured for 48 h and conditioned media assumed to represent the secretome from primed MSCs. This primed MSC secretome was then placed on (a) untreated or (b) IL-1 β-stimulated chondrocytes. a Conditioned media from IFN-γ-primed MSCs induced downregulation in ACAN and upregulation of CXCL10 compared to untreated control chondrocytes. b In IL-1β-stimulated chondrocytes, conditioned media from IFN-γ-primed MSCs also induced downregulation in ACAN and upregulation of CXCL10 compared to untreated control chondrocytes. All conditioned media groups induced downregulation of IL-6 and PTGS2 genes. Box and whisker plots represent n = 8 individual horses. *Group significantly different from control IL-1β-stimulated chondrocyte gene expression based on a 95% confidence interval. MSC mesenchymal stem cell, poly I:C polyinosinic:polycytidylic acid, IFN-γ interferon gamma, ACAN aggrecan, MMP-13 collagenase 3, PTGS2 prostaglandin-endoperoxide synthase 2, CXCL10 C-X-C motif chemokine 10, CCL2 C-C motif chemokine 2, IL interleukinBack to article page