Fig. 2
Isolation, cultivation and characterization of rat adipose-derived mesenchymal stem cells (AD-MSCs). A Morphology of AD-MSCs (rat AD-MSCs displayed spindle-shaped morphology, ×100). B Immunophenotype of AD-MSCs. Immunophenotype of rat AD-MSCs analyzed by flow cytometry. Most cells expressed CD44, CD73 and CD90, but were CD45-negative and CD11b-negative. C Multipotency of AD-MSCs. Rat AD-MSCs could differentiate into adipogenic (c1, ×200), osteogenic (c2, ×100) and chondrogenic (c3, ×200) lineages when cultured in differentiation medium. FITC fluorescein isothiocyanate, PE phycoerythrin