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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages

Fig. 2

Differentiation of hiPSCs into RPE cells. a Schematic of differentiation of hiPSCs into RPE cells and time points of sampling for microarray analysis. b Bright-field image of hiPSC-derived RPE cells, showing melanin pigmentation. c Western blot analysis demonstrating expression of positive RPE markers in hiPSC-derived RPE cells. d Expression of positive RPE markers demonstrated by immunofluorescent microscopy. Nuclei counterstained with DAPI, and merged DAPI and immunofluorescent signals shown. IGF insulin-like growth factor, bFGF basic fibroblast growth factor, hiPSC human induced pluripotent stem cell, RPE retinal pigment epithelium

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