Fig. 5

Differentially expressed transcription factors (TFs). Venn diagrams demonstrate numbers of common and unique differentially overexpressed (a) and underexpressed (b) TF-encoding genes in OV, RGC and RPE lineages compared to original hiPSCs according to criteria of fold-change > 2 and p < 0.01. Top 10 most highly differentially expressed TF-encoding genes with respective fold-changes shown in text boxes. c Flowchart demonstrating selection of 57 most highly regulated TF-encoding genes with contrast expression patterns between samples. d Hierarchical clustering of the selected 57 TF-encoding genes. Three clusters (1, 2.1 and 2.2) described in Results are labeled red. OV optic vesicle, RGC retinal ganglion cell, RPE retinal pigment epithelium, FC fold-change, DEG differentially expressed gene, AnimalTFDB Animal Transcription Factor Database