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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

Fig. 8

Confocal laser scanning microscopy analysis. a Confocal contrast image (left panel 1) of 3D-PLA (gray) grafted in mice calvaria after 6 weeks of implantation, with the corresponding 3D reconstruction image (right panel 2). b 3D-PLA (gray) and PKH-67-stained EVs (green) after 6 weeks of in vivo implantation, with the corresponding 3D reconstruction image. c 3D-PLA (gray) and PKH-67-stained PEI-EVs (green), with the corresponding 3D reconstruction image. d 3D-PLA (gray) and PKH-26-stained hGMSCs (red), with the corresponding 3D reconstruction image. e 3D-PLA (gray), PKH-26-stained hGMSCs (red), and PKH-67-stained EVs, with the corresponding 3D reconstruction image. f 3D-PLA (gray), PKH-26-stained hGMSCs (red), and PKH-67-stained PEI-EVs, with the corresponding 3D reconstruction image. Scale bars = 20 μm

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