Fig. 10

Reversal of suppressive effect of hPL on myogenic potential of hMuStem cells. hMuStem cells^hPL cultured up to P3 in appropriate GM and then passaged to HS-GM or heparin-free hPL-GM (hPL^fd or hPL^gel), then myogenic differentiation assessed. a sMyHC immunolabeling performed after 4 days of culture in fusion-promoting low-serum medium. Nuclei counterstained with DRAQ5 (blue). Scale bar, 100 μm. b Fusion index (FI) in six independent cell batches. c Western blot analyses of sMyHC and GAPDH protein abundance in hMuStem cells^HS maintained in HS-GM, as well as hMuStem cells^hPL cultured with either hPL-GM or HS-GM. Data presented as mean ± SEM (^ap < 0.05, ^cp < 0.001 versus HS; *p < 0.05, **p < 0.01, ***p < 0.001; LME model followed by Tukey’s post-hoc test). HS human serum, hPL human platelet lysate, hPL^gel heparin-free hPL that was allowed to form a nutritive gel over the cell layer, hPL^fd heparin-free fibrinogen-depleted hPL, sMyHC sarcomeric myosin heavy chain, GAPDH glyceraldehyde 3-phosphate dehydrogenase