Fig. 3

Phenotypic profile of hMuStem cells^HS and hMuStem cells^hPL. Flow cytometry comparison of (a) mesenchymal (CD29, CD44, CD73, CD90 and CD105) and (b) hematopoietic (CD34, CD45) and endothelial (CD144) cell lineage markers in hMuStem cells^HS and hMuStem cells^hPL. Results of one representative donor out of three independent donors presented. Myogenic (CD56) and perivascular (CD140b and CD146) cell lineage markers analyzed either in (c) entire viable cell population or (d) CD56⁺ cell subsets. Results presented for three independent donors: when significant but below 100, percentage of positive cells indicated in upper right corner of corresponding histograms. e Immunocytochemistry analysis of myogenic regulatory factors (MRFs) Myf5, MyoD, and desmin in hMuStem cells^HS and hMuStem cells^hPL at P4. Representative pictures presented. Nuclei counterstained with DAPI (blue). Proportions of positive cells recorded for three independent donors presented. Donors #4, #5, and #6 represented by squares, triangles, and circles, respectively. Data presented as mean ± SD for each donor. Scale bar, 100 μm. HS human serum, hPL human platelet lysate