Fig. 5

Myogenic differentiation potential of hMuStem cells^HS and hMuStem cells^hPL. a Myogenic differentiation potential of hMuStem cells isolated and expanded either with HS (hMuStem cells^HS) or hPL (hMuStem cells^hPL) determined at P4 (n = 3, donors #4–#6). Cells expressing myogenic markers MyoD and myogenin counted and fusion index (FI) calculated as percentage of nuclei identified in sarcomeric myosin heavy chain-positive (sMyHC⁺) myotubes. b Contribution of hMuStem cells to myogenesis evaluated in coculture experiments with mouse D7 cells. Number of human lamin A/C⁺ nuclei (arrow) counted within hybrid sMyHC⁺ myotubes. Nuclei counterstained with DAPI or DRAQ5 (blue). Donors #4, #5, and #6 represented by squares, triangles, and circles, respectively. Data presented as mean ± SD for each donor. Scale bar, 100 μm. HS human serum, hPL human platelet lysate