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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression

Fig. 2

Detection and quantification of topoisomerase (topo) IIβ overexpression and silencing in hMSCs. a Topo IIβ was silenced using 7.5, 10, 12.5, 15, and 20 nM topo IIβ-specific small interfering (si)RNAs and silencing efficiency was determined by RT-qPCR. b siRNA-based cytotoxicity for each siRNA concentration was evaluated by MTT cell proliferation assay. c Topo IIβ was overexpressed using 4, 5, 6, 8, and 10 μg pEGFP-topo IIβ and transfection efficiency was quantified by RT-qPCR. d EGFP-topo IIβ plasmid-based cytotoxicity for each plasmid concentration was evaluated by MTT cell proliferation assay. e Morphology of control (untransfected hMSCs), topo IIβ-silenced, and -overexpressed hMSCs. f Topo IIβ expression levels of topo IIβ-silenced (10 nm siRNA) and -overexpressed (6 μg pEGFP-topo IIβ) hMSCs were compared by RT-qPCR. Images ×10. Error bars represent the means ± standard deviation; *p < 0.01, **p < 0.001. NS, nonsignificant

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Stem Cell Research & Therapy

ISSN: 1757-6512