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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p

Fig. 6

MiR-199a 5p downregulated the expression of VEGFA. Alterations of VEGFA in different experimental groups were detected after miR-199a-5p transfection or knockdown under normoxia and H/SD condtions. a Potential consequential paring of VEGFA-targeted region and miR-199a-5p. b Expression of miR-199a-5p detected by qRT-PCR. c, d Western blot analysis of the protein expression of VEGFA. e qRT-PCR analysis of the mRNA expression of VEGFA. MSCs+miR-199a-5p, MSCs transfected with miR-199a-5p mimics; MSCs+miR-199a-5p NC, MSCs transfected with miR-199a-5p mimics negative control; MSCs+miR-199a-5p inhibitor, MSCs transfected with miR-199a-5p inhibitor; MSCs+miR-199a-5p inhibitor NC, MSCs transfected with miR-199a-5p inhibitor negative control. **P < 0.01 vs MSCs, ##P < 0.01vs miR-199a-5p NC, ††P < 0.01 vs miR-199a-5p inhibitor NC. H/SD: hypoxia/serum deprivation, MSCs: mesenchymal stem cells, NC: scramble negative control, UTR: untranslated region, VEGFA: vascular endothelial growth factor A

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