Fig. 3

ATP6AP2 signaling is dependent on MAPK in hADSCs. hADSCs isolated from healthy and ND subjects and differentiated for 37 days. Cell lysates (80–150 μg of protein) separated by SDS-PAGE and immunoblotted with antibodies raised against p-ERK and total ERK. Proteins expression determined during differentiation from day < 0 to day 37 (a) or in NLCs at day 37 to evaluate the renin responsiveness (b). NLCs at day 37 treated after serum starvation overnight with human recombinant renin (hRenin, 50 nM) for indicated time course with or without 30 μM PD98059; to prevent any ERK phosphorylation potentially due to ANG II generation, Losartan (10 μM) and PD123319 (10 μM) ANG II blockers added during time course; quantitative variations in total and p-ERK amounts of p42 and p44 combined and shown. *^§P < 0.05,**^§§&P < 0.01,***P < 0.005: *D0/14/30/37 vs day < 0 or hRenin T10/30/60 vs T = 0, ^§ND vs healthy, ^&PD98059-treated vs untreated cells. D day, ERK extracellular signal-regulated kinase, ND neurodegenerative disease, T time