Fig. 6

Subcellular distribution of ATP6AP2 proteins. Subcellular distribution of ATP6AP2 protein in cells derived from healthy (a–c) and ND (d–f) subjects examined during neurogenic differentiation of hADSCs during 37 days of differentiation. Lysate proteins (50–100 μg) separated by SDS-PAGE and immunoblotted with polyclonal antibodies directed against full length of ATP6AP2, N-20 recognizes CAV-1 and A-19 epitope mapping within internal region of FLOT-2. Subcellular distribution of ATP6AP2 determined in cellular (total lysates), plasma membrane (PM), caveolae/lipid raft membrane microdomain (CLR-M), postnuclear supernatant (PNS), endoplasmic reticulum (ER), Golgi, lysosome and nucleus. Expression of CAV (g) and FLOT (h) proteins during differentiation of healthy ADSCs. *P < 0.05,**P < 0.01;***P < 0.0005: D0/14/30/37 vs day < 0. CAV caveolin, D day, FLOT flotillin, ND neurodegenerative disease