Fig. 1

Initially attached heterogeneous AFCs display homogeneous cell morphology upon passaging and include typical kidney cell morphologies. Phenotypical complexity of AFCs observed, regardless of culture media composition within first two passages. Upon further passaging, cells became homogeneous with fibroblast-like morphology (a). During culture, subpopulation of cells observed which had similar morphology to distinct renal-originated progenitor cells UM51 and HREpCs (b). AF-MSC amniotic fluid mesenchymal stem cell, HREpC human renal epithelial cell, αMEM minimum essential medium alpha modification, RCM renal cell medium