Fig. 4

CXCL12-EPC transplantation promoted neurogenesis in ischemic mouse brain. A (a) BrdU staining and (b) quantification of BrdU⁺ cells in perifocal area at 5 weeks after MCAO. B (a) Double immunostaining of DCX and BrdU-positive cells and (b) quantification of DCX⁺/BrdU⁺ cells in the SVZ at 5 weeks after MCAO. C (a) Double immunostaining of DCX and BrdU cells (white arrows) and (b) quantification of DCX⁺/BrdU⁺ cells in perifocal region of ipsilateral hemisphere at 5 weeks after MCAO. D (a) Double immunostaining of NeuN and BrdU (white arrows indicate double positive cells) and (b) quantification of NeuN⁺/BrdU⁺ cells in perifocal region of ipsilateral hemisphere 5 weeks after MCAO. n = 3–4 mice/group. Scale bar: 50 μm. Data presented as mean ± SD. *p < 0.05, **p < 0.01. All markers presented by pseudo colors. PBS phosphate-buffered saline, GFP-EPC endothelial progenitor cell modified by gfp gene, GFP green fluorescent protein, CXCL12-EPC endothelial progenitor cell modified by cxcl12 gene, SVZ subventricular zone