Fig. 5

CXCL12-EPC transplantation protected myelin sheath integrity in ischemic mouse brain. A (a) DAB immunostaining of MBP-positive cells and (b) IOD semiquantification of MBP⁺ signal in ipsilateral hemisphere at 5 weeks after MCAO. B (a) DAB immunostaining of PDGFRα-positive cells in SVZ and perifocal area and quantification of PDGFRα⁺ cells in (b) SVZ and (c) perifocal area in ipsilateral hemisphere at 5 weeks of ischemic mouse brain. Insets are higher magnifications from corresponding images. n = 4 mice/group. Scale bar: 100 μm. Data presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. MBP myelin basic protein, PBS phosphate-buffered saline, GFP-EPC endothelial progenitor cell modified by gfp gene, GFP green fluorescent protein, CXCL12-EPC endothelial progenitor cell modified by cxcl12 gene, IOD integral optical density, PDGFR platelet-derived growth factor receptor, OPC oligodendrocyte progenitor cell, SVZ subventricular zone