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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation

Fig. 2

Derivation of hESCs by MTP. a On day 2 after ICM isolation, prominent ICM clump (arrowheads) surrounded with proliferating TE cells observed. Under feeder-free conditions using either CELLstart or Matrigel, most proliferating TE cells were degenerated on culture day 3 (arrows) while remaining ICM clump was transferred to fresh HFFs for further expansion. b Procedure of hESC derivation by MTP method. Scale bar: 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512