Fig. 3

Derivation of hESCs from aneuploid embryos using MTP under a feeder-free system. a Eight-cell-stage human embryos subjected to blastomere biopsy and their chromosomal abnormalities determined by fluorescence in-situ hybridization (FISH). b Mechanically isolated ICM (arrowheads) with large number of associated TE cells (arrows) detached from Matrigel-coated surface and degenerated after 4 days of culture. c Mechanically isolated ICM (arrowheads) carefully stripped of TE cells (arrows) remains attached to Matrigel-coated surface and continuously proliferated to form large hESC colonies while remaining TE cells degenerated. Scale bar: 50 μm