Fig. 8
NO and the JNK signaling pathway work to the same effect in regular medium compared with induced medium. a Real-time RT-PCR showing that the expression level of osteopontin (OPN) and osterix (OSX) were significantly increased by sodium nitroprusside (SNP) in PDLSCs in regular medium, while SP600125 could eliminate this effect. Blocking NO generation by l-NG-monomethyl arginine (l-NMMA) in PDLSCs in regular medium led to downregulation of OPN and OSX, and anisomycin could reverse this influence. All experiments are representative of three replicates. **P < 0.01. b Real-time RT-PCR showing that the expression level of lipoprotein lipase (LPL) and CCAAT-enhancer binding protein (C/EBP)α were significantly decreased by SNP in PDLSCs in regular medium, while SP600125 could eliminate this effect. Blocking NO generation by l-NMMA in PDLSCs in regular medium led to upregulation of LPL and C/EBPα, and anisomycin could reverse this influence. All experiments are representative of three replicates. **P < 0.01. c Western blot indicating that alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2) levels were significantly increased by SNP in PDLSCs in regular medium, while the expression of peroxisome proliferator-activated receptor (PPAR)γ was inhibited. Blocking the JNK signaling pathway was able to reverse the influence induced by NO. When the NO level was downregulated by l-NG-monomethyl arginine (l-NMMA) in PDLSCs in regular medium, the expression of ALP and Runx2 were downregulated and PPARγ was upregulated. Activating the JNK signaling pathway was able to reverse the influence of l-NMMA