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Table 1 Primers used for real-time RT-PCR

From: Negative effects of a high tumour necrosis factor-α concentration on human gingival mesenchymal stem cell trophism: the use of natural compounds as modulatory agents

Gene Primer nucleotide sequences Product size (base pairs) Annealing temperature
CD34 Forward: 5’-GCCTGGAGCAAAATAAGACCTC-3’
Reverse: 5’-AGGATCCCCAGCTTTTTCAGG-3′
250 bp 55 °C
CD45 Forward: 5’-TGTGGAGCCAATCCATGCAGA-3’
Reverse: 5’-GTTTGACCCTGCATCTCCGTT-3′
232 bp 55 °C
CD90 Forward: 5’-CACACATACCGCTCCCGAA-3’
Reverse: 5’-CACCAGTCACAGGGACATGAA-3′
279 bp 55 °C
CD105 Forward: 5’-ATACCACTAGCCAGGTCTCGAA-3’
Reverse: 5’-ATGGCAGCTCTGTGGTGTTG-3′
288 bp 55 °C
NF-kB Forward: 5′-CAGCAGATGGCCCATACCTT-3′
Reverse: 5′-CACCATGTCCTTGGGTCCAG-3′
287 bp 55 °C
mTOR Forward: 5′-GCCCCTACATGGAGCCTATTC-3′
Reverse: 5′-CCTGGAGCATGTCCATGATGA-3′
183 bp 55 °C
Oct4 Forward: 5’-CTCACCCTGGGGGTTCTATT-3’
Reverse: 5’-CTCCAGGTTGCCTCTCACTC-3’
230 bp 55 °C
SOX2 Forward: 5′-CATGAAGGAGCACCCGGATT-3′
Reverse: 5′-ATGTGCGCGTAACTGTCCAT-3′
186 bp 55 °C
TNF-α Forward: 5′-AGGGACCTCTCTCTAATCAGCC-3′
Reverse: 5′-GCTTGAGGGTTTGCTACAACA-3′
101 bp 55 °C
IL-6 Forward: 5′-TCCTCGACGGCATCTCA-3′
Reverse: 5′-TTTTCACCAGGCAAGTCTCCT-3′
165 bp 55 °C
COX-2 Forward: 5′-TGTGTTGACATCCAGATCACAT-3′
Reverse: 5′-GGAGTCGGGCAATCATCAGG-3′
237 bp 55 °C
IL-10 Forward: 5′-CAAGCTGAGAACCAAGACCC-3′
Reverse: 5′-AAGATGTCAAACTCACTCATGGC-3′
141 bp 55 °C
TGF-β Forward: 5′-ACTGCAAGTGGACATCAACG-3′
Reverse: 5′-TGCGGAAGTCAATGTACAGC-3′
218 bp 55 °C
β-actin Forward: 5’-GCACTCTTCCAGCCTTCCTTCC-3’
Reverse: 5’-GAGCCGCCGATCCACACG-3’
254 bp 55 °C