Table 1 Primers used for real-time RT-PCR
Gene | Primer nucleotide sequences | Product size (base pairs) | Annealing temperature |
|---|---|---|---|
CD34 | Forward: 5’-GCCTGGAGCAAAATAAGACCTC-3’ Reverse: 5’-AGGATCCCCAGCTTTTTCAGG-3′ | 250 bp | 55 °C |
CD45 | Forward: 5’-TGTGGAGCCAATCCATGCAGA-3’ Reverse: 5’-GTTTGACCCTGCATCTCCGTT-3′ | 232 bp | 55 °C |
CD90 | Forward: 5’-CACACATACCGCTCCCGAA-3’ Reverse: 5’-CACCAGTCACAGGGACATGAA-3′ | 279 bp | 55 °C |
CD105 | Forward: 5’-ATACCACTAGCCAGGTCTCGAA-3’ Reverse: 5’-ATGGCAGCTCTGTGGTGTTG-3′ | 288 bp | 55 °C |
NF-kB | Forward: 5′-CAGCAGATGGCCCATACCTT-3′ Reverse: 5′-CACCATGTCCTTGGGTCCAG-3′ | 287 bp | 55 °C |
mTOR | Forward: 5′-GCCCCTACATGGAGCCTATTC-3′ Reverse: 5′-CCTGGAGCATGTCCATGATGA-3′ | 183 bp | 55 °C |
Oct4 | Forward: 5’-CTCACCCTGGGGGTTCTATT-3’ Reverse: 5’-CTCCAGGTTGCCTCTCACTC-3’ | 230 bp | 55 °C |
SOX2 | Forward: 5′-CATGAAGGAGCACCCGGATT-3′ Reverse: 5′-ATGTGCGCGTAACTGTCCAT-3′ | 186 bp | 55 °C |
TNF-α | Forward: 5′-AGGGACCTCTCTCTAATCAGCC-3′ Reverse: 5′-GCTTGAGGGTTTGCTACAACA-3′ | 101 bp | 55 °C |
IL-6 | Forward: 5′-TCCTCGACGGCATCTCA-3′ Reverse: 5′-TTTTCACCAGGCAAGTCTCCT-3′ | 165 bp | 55 °C |
COX-2 | Forward: 5′-TGTGTTGACATCCAGATCACAT-3′ Reverse: 5′-GGAGTCGGGCAATCATCAGG-3′ | 237 bp | 55 °C |
IL-10 | Forward: 5′-CAAGCTGAGAACCAAGACCC-3′ Reverse: 5′-AAGATGTCAAACTCACTCATGGC-3′ | 141 bp | 55 °C |
TGF-β | Forward: 5′-ACTGCAAGTGGACATCAACG-3′ Reverse: 5′-TGCGGAAGTCAATGTACAGC-3′ | 218 bp | 55 °C |
β-actin | Forward: 5’-GCACTCTTCCAGCCTTCCTTCC-3’ Reverse: 5’-GAGCCGCCGATCCACACG-3’ | 254 bp | 55 °C |