Fig. 4

Antiviral efficacy against HSV-1 lytic infection in A-3D neuronal cultures as determined by flow cytometry and CX7 HCS technology. We utilized a genetically engineered HSV-1 construct, incorporating enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) as reporter genes, whose expression is driven by the viral promoters ICP0 and glycoprotein C, respectively. The images show confocal microscopy analysis of HSV-1-infected A-3D neuronal cultures depicting the EGFP (a) and RFP (b) reporter genes. Cell nuclei are counterstained with TO-PRO-3 (TOPRO), (c). The graphs show the determination of IC50 for acyclovir in HSV-1-infected A-3D neuronal culture by flow cytometry and CX7 HCS technology (left), and determination of IC50 for acyclovir in HSV-1-infected 2D neuronal cultures by flow cytometry (right). The data represent an average of three independent experiments. Error bars represent model-based standard error