Cellular therapy with human autologous adipose-derived adult cells of stromal vascular fraction for alopecia areata

Table 1 Immunophenotyping and apoptotic index of cell surface markers expressed by total nucleated SVF cells

ADSVCs (SVF cells) Freshly isolated from adipose tissue	% of total cells
CD13⁽⁺⁾	71.06 ± 5.08
CD14⁽⁺⁾	10.28 ± 4.11
CD29⁽⁺⁾	32.44 ± 2.09
CD31⁽⁺⁾	18.56 ± 2.83
CD34⁽⁺⁾	71.27 ± 3.54
CD45⁽⁺⁾	27.70 ± 6.33
CD73⁽⁺⁾	27.07 ± 5.81
CD90⁽⁺⁾	55.27 ± 4.16
CD105⁽⁺⁾	6.37 ± 4.12
CD144 (VE cadherin)⁽⁺⁾	18.62 ± 4.92
CD146⁽⁺⁾	24.45 ± 2.17
CD166⁽⁺⁾	3.19 ± 2.05
HLA-ABC⁽⁺⁾	98.04 ± 1.12
HLA-DR⁽⁺⁾	10.17 ± 3.62
Annexin V⁽⁻⁾/PI⁽⁻⁾/7AAD⁽⁻⁾ (viable cells)	98.97 ± 0.71
Annexin V⁽⁺⁾/PI⁽⁻⁾/7AAD⁽⁻⁾ (early-apoptotic cells)	1.02 ± 0.54
Annexin V⁽⁺⁾/PI⁽⁺⁾/7AAD⁽⁺⁾ (late apoptotic, necrotic cells)	0.17 ± 0.82

2 × 10⁵ cells were labeled with fluorescence-coupled antibodies against the indicated cell surface markers, Annexin V, 7AAD, propidium iodide solution (PI), and analyzed using a MACSQuant flow analyzer as indicated in Materials and Methods. The results are expressed as the mean ± SEM of all subjects (n = 20) each performed in duplicate
ADSVC adipose-derived stromal vascular cell, SVF stromal vascular fraction

ISSN: 1757-6512