Fig. 2

hES-AS take up glutamate from medium. a Glutamate concentration measured in solutions with 500 μM glutamate that were incubated for indicated times either alone (black bars 1–2) or with hES-AS differentiated for 28 days (black bars 3–7). Kinetics of glutamate removal by hES-AS similar to that by astrocytes extracted from human spinal cord (gray bars). b Percentage of glutamate uptake after 60 min by hES-AS alone or in presence of inhibitors of glutamate transporter GLT-1, WAY-213,613 (1 μM) and DHK (500 μM). Error bars are SD of triplicates. *p < 0.05. hESC human embryonic stem cell, DHK dihydrokainic acid